Collecting Fractions Automatically (Autopurification)
Autopurification Samples in the Chromatogram

After Autopurification has been performed, you can have Chromeleon display the results of the different samples in the associated chromatograms. The default setting is that all samples of the Analytic, Preparation, and Fraction Auto Purification Types that belong together are displayed:

The topmost chromatogram shows the original analytical sample. If this sample fulfills the conditions determined in the Create Purification Samples Post-Acquisition Steps, a preparation-type sample is created and fractionated. The chromatogram for the preparation-type sample is displayed next. The vertical stripes indicate the chromatogram ranges for which fractions were collected.

The individual fractions are chromatographically reanalyzed, based on the conditions determined in the Create Fraction Analysis Samples post-acquisition step. The chromatogram for the associated fractions is displayed last.

The bottommost chromatogram shows the fraction for the selected peak of the preparative sample. This is the fraction under the peak maximum. Of, if no fraction was collected at the peak maximum, this is the fraction overlapping most with the peak. If no peak was selected in the preparative chromatogram or if no fraction was collected for the selected peak, the chromatogram for the first peak is displayed.

 Note:  

To display the next or previous sample or channel of the current chromatogram, click the associated icon: Click for the next chromatogram, for the previous chromatogram, for the next channels or for the previous channel. If another chromatogram is displayed, the associated chromatograms are updated simultaneously. For example, if Chromeleon displays the next analytical chromatogram, the associated preparative chromatogram and the associated chromatogram of the first fraction are displayed as well.

To change the representation of the single chromatograms, double-click the chromatogram or select Decoration on the context menu. The Chromatogram Decoration dialog box is opened. On the Extended Fractionation tab page, determine which autopurification samples are displayed. On the Fractions tab page, determine how fraction collection is displayed in the chromatogram.

Also, refer to  Autopurification Samples in the Tray Views.

For an overview of how to perform autopurification, refer to  Collecting Fractions Automatically (Autopurification).